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250 大学院医歯学総合研究科(医) = Graduate School of Medical and Dental Sciences (Medical) >
10 学術雑誌論文 = Journal Article >
10 査読済論文 = Postprint >


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Title :The effect of hydrodynamic-based delivery of an interleukin-13-Ig fusion gene for experimental autoimmune myocarditis in rats and its possible mechanism
Authors :Elnaggar, Raafat
Hanawa, Haruo
Liu, Hui
Yoshida, Tsuyoshi
Hayashi, Manabu
Watanabe, Ritsuo
Abe, Satoru
Toba, Ken
Yoshida, Kaori
Chang, He
Minagawa, Shiro
Okura, Yuji
Kato, Kiminori
Kodama, Makoto
Maruyama, Hiroki
Miyazaki, Junichi
Aizawa, Yoshifusa
Issue Date :2005
Journal Title :European journal of immunology
Volume :35
Issue :6
Start Page :1995
End Page :2005
ISSN :0014-2980
Description :Interleukin (IL)-13 is a pleiotropic cytokine secreted by activated Th2-T lymphocytes. Th1 cytokines are assumed to exacerbate while Th2 cytokines to ameliorate rat experimental autoimmune myocarditis (EAM). Here, we examined the effect of IL-13 on EAM using a hydrodynamic-based delivery of an IL-13-Ig and the possible mechanism of its effect. Rats were immunized on day 0 and IL-13-Ig treated rats were injected with pCAGGS-IL-13-Ig and control rats with pCAGGS-Ig on day 1 or 7. On day 17, IL-13-Ig gene therapy was effective in controlling EAM as monitored by the decreased heart weight/body weight ratio, reduced myocarditis and atrial natriuretic peptide mRNA in heart as a heart failure marker. On the basis of IL-13 receptor mRNA expression in separated cells from EAM hearts, we proposed that IL-13-Ig targeting cells were CD11b+ cells and non-cardiomyocytic non-inflammatory (NCNI) cells such as fibroblasts, smooth muscle or endothelial cells. IL-13-Ig inhibited expressing the genes of prostaglandin E synthase, cyclooxygenase-2, inducible nitric oxide synthase, IL-1β and TNFα of cultivated cells from EAM hearts in contrast, while it enhanced IL-1 receptor antagonist. We concluded that IL-13-Ig ameliorates EAM and supposed that its effectiveness may be due to the influence on these immunologic molecules in CD11b+ and NCNI cells.
Keywords :myocarditis
dilated cardiomyopathy
immune system
gene therapy
Type Local :学術雑誌論文
Language :eng
URI :http://hdl.handle.net/10191/1159
fullTextURL :http://dspace.lib.niigata-u.ac.jp/dspace/bitstream/10191/1159/1/22_0003.pdf
DOI :info:doi/10.1002/eji.200425776
Rights :WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Appears in Collections:10 査読済論文 = Postprint

Please use this identifier to cite or link to this item: http://hdl.handle.net/10191/1159