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Title :Resting spore of plasmodiophora brassicae proliferates only in the callus of clubroot disease-susceptible turnip but increases the PAL activity in the callus of clubroot disease-resistant turnip
Authors :Takahashi, Hideyuki
Muraoka, Satoko
Ito, Kimiko
Mitsui, Toshiaki
Hori, Hidetaka
Kiso, Akira
Publisher :日本植物細胞分子生物学会
Issue Date :Sep-2001
Journal Title :Plant Biotechnology
Volume :18
Issue :4
Start Page :267
End Page :274
ISSN :1342-4580
Abstract :The calluses were indeced from turnips, Brassicae campestris L. which were susceptible and resistant to Plasmodiophora brassicae in Murashige-Skoog’s agar medium supplemented with 1.0 ppm 6-benzylaminopurine and 0.5 ppm α-naphthalene acetic acid. When a 10 μl water suspension containing 104 resting spores of P. brassicae was placed on the surface of the calluses, about 3 × 105 zoosporangium-like spheroids (SLS) were recovered from the susceptible calluses after 24 h of the treatment but no SLS was found in the resistant callus. On 6th day after the treatment, the SLS increased to about 4 × 106 in the susceptible callus. Upon inoculation of resistant callus with 104 resting spores, the phenylalanine ammonia lyase (PAL) activity increased about 4-fold after 20 h, however, such increase was not observed in the susceptible callus during the same period. The consitutive PAL activity of susceptible callus was roughly one 6th of that of resistant callus.
Keywords :Brassicae campestris L.
callus
clubroot discase resistance and susceptible
PAL
phenylalanine ammonia lyase
Plasmodiophora brassicae
resting spore
turnip
Type Local :学術雑誌論文
Language :eng
Format :application/pdf
URI :http://hdl.handle.net/10191/17592
fullTextURL :http://dspace.lib.niigata-u.ac.jp/dspace/bitstream/10191/17592/1/ZL_18(4)_267-274.pdf
Appears in Collections:10 査読済論文 = Postprint

Please use this identifier to cite or link to this item: http://hdl.handle.net/10191/17592