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260 大学院医歯学総合研究科(歯) = Graduate School of Medical and Dental Sciences (Dental) >
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タイトル :Oral Administration of P. gingivalis Induces Dysbiosis of Gut Microbiota and Impaired Barrier Function Leading to Dissemination of Enterobacteria to the Liver
別言語のタイトル :P. gingivalis口腔投与によって腸内細菌叢の撹乱と腸管透過性機能異常が誘導され腸内細菌の肝臓への播種が亢進する
著者 :Nakajima, Mayuka
公開者・出版者 :新潟大学
刊行年月 :2016-3-23
内容記述 :学位の種類: 博士(歯学). 報告番号: 甲第4147号. 学位記番号: 新大院博(歯)甲第345号. 学位授与年月日: 平成28年3月23日
PLoS One. 2015, 10(7), e0134234
抄録 :Although periodontitis has been implicated as a risk factor for various systemic diseases, the precise mechanisms by which periodontitis induces systemic disease remain to be elucidated. We have previously revealed that repeated oral administration of Porphyromonas gingivalis elicits endotoxemia via changes in the gut microbiota of the ileum, and thereby induces systemic inflammation and insulin resistance. However, it is not clear to what extent a single administration of P. gingivalis could affect gut microbiota composition, gut barrier function, and subsequent influx of gut microbiota into the liver. Therefore, in the present study, C57BL/6 mice were orally administered P. gingivalis (strain W83) once and compared to sham-inoculated mice. The phylogenetic structure and diversity of microbial communities in the gut and liver were analyzed by pyrosequencing the 16S ribosomal RNA genes. Serum endotoxin activity was determined by a Limulus amebocyte lysate test. Gene expression in the intestine and expression of 16S rRNA genes in the blood and liver were examined by quantitative polymerase chain reaction. Administration of P. gingivalis significantly altered gut microbiota, with an increased proportion of phylum Bacteroidetes, a decreased proportion of phylum Firmicutes, and increased serum endotoxin levels. In the intestinal tissues, gene expression of tjp-1 and occludin, which are involved in intestinal permeability, were downregulated. Higher amounts of bacterial DNA were detected in the liver of infected mice. Importantly, changes in gut microbiota preceded systemic inflammatory changes. These results further support the idea that disturbance of the gut microbiota composition by orally derived periodontopathic bacteria may be a causal mechanism linking periodontitis and systemic disease.
資料種別 :学位論文
言語 :eng
フォーマット :application/pdf
application/pdf
URI :http://hdl.handle.net/10191/42133
fullTextURL :http://dspace.lib.niigata-u.ac.jp/dspace/bitstream/10191/42133/1/h27ndk345_a.pdf
http://dspace.lib.niigata-u.ac.jp/dspace/bitstream/10191/42133/2/h27ndk345.pdf
DOI :info:doi/10.1371/journal.pone.0134234
権利 :Copyright: © 2015 Nakajima et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
学位授与番号 :13101甲第4147号
学位授与年月日 :2016-03-23
学位名 :博士(歯学)
学位授与機関 :新潟大学
出現コレクション:10 博士学位論文

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