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Title :Proteomic Identification of α-Amylase Isoforms Encoded by RAmy3B/3C from Germinating Rice Seeds
Authors :Nanjo, Yohei
Asatsuma, Satoru
Itoh, Kimiko
Hori, Hidetaka
Mitsui, Toshiaki
Publisher :Japan Society for Bioscience, Biotechnology, and Agrochemistry
Issue Date :2004
Journal Title :Bioscience, Biotechnology, and Biochemistry
Volume :68
Issue :1
Start Page :112
End Page :118
ISSN :0916-8451
Description :We isolated and identified 10 α-amylase isoforms by using β-cyclodextrin Sepharose affinity column chromatography and two-dimensional polyacrylamide gel electrophoresis from germinating rice (Oryza sativa L.) seeds. Immunoblots with anti-α-amylase I-1 and II-4 antibodies indicated that 8 isoforms in 10 are distinguishable from α-amylase I-1 and II-4. Peptide mass fingerprinting analysis showed that there exist novel isoforms encoded by RAmy3B and RAmy3C genes. The optimum temperature for enzyme reaction of the RAmy3B and RAmy3C coding isoforms resembled that of α-amylase isoform II-4 (RAmy3D). Furthermore, complex protein polymorphism resulted from a single α-amylase gene was found to occur not only in RAmy3D, but also in RAmy3B.
Keywords :α-amylase
germinating rice seed
RAmy3B
RAmy3C
proteomics
Type Local :学術雑誌論文
Language :eng
URI :http://hdl.handle.net/10191/6326
fullTextURL :http://dspace.lib.niigata-u.ac.jp/dspace/bitstream/10191/6326/1/07_0002.pdf
Appears in Collections:10 査読済論文 = Postprint

Please use this identifier to cite or link to this item: http://hdl.handle.net/10191/6326