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01 Acta medica et biologica >


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Title :Protective effects of free radical scavengers on transient ischemia-induced retinal ganglion cell death
Authors :Okuyama, Shinya
Publisher :Niigata University School of Medicine
Issue Date :Jun-2011
Journal Title :Acta medica et biologica
Volume :58
Issue :1
Abstract :Objective: To evaluate the suppressive effect of two free radical scavengers, edaravone (3-methyl-1-phenyl-2-pyrazolin-5-one) and Trolox® (6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid), on retinal ganglion cell death in transgenic Thy1-EGFP mice expressing enhanced green fluorescent protein in retinal ganglion cells. / Method: Retinal ischemia-reperfusion injury was performed on mice following intraperitoneal administration of edaravone or Trolox, and the number of retinal ganglion cells that survived ischemia/reperfusion was measured non-invasively over time using fluorescent fundus angiography. In addition, viable cells were counted in flat mount retinal specimens prepared from enucleated eyes. / Results: The fluorescence intensity at the fundus decreased steadily after transient ischemia/reperfusion. Administration of edaravone and Trolox suppressed this decrease by approximately 20% on day 7. These results were comparable with the reduction in the number of ganglion cells counted in flat mount retinal preparations. / Conclusion: Free radical scavengers effectively suppress ischemia-induced retinal ganglion cell death.
Keywords :Reactive oxygen spieces
Type Local :紀要論文
Language :eng
Format :application/pdf
URI :http://hdl.handle.net/10191/19343
fullTextURL :http://dspace.lib.niigata-u.ac.jp/dspace/bitstream/10191/19343/1/Vol.58 No.1-2 Okuyama.pdf
Appears in Collections:01 Acta medica et biologica

Please use this identifier to cite or link to this item: http://hdl.handle.net/10191/19343